16 research outputs found
Experimental design trade-offs for gene regulatory network inference: an in silico study of the yeast Saccharomyces cerevisiae cell cycle
Time-series of high throughput gene sequencing data intended for gene
regulatory network (GRN) inference are often short due to the high costs of
sampling cell systems. Moreover, experimentalists lack a set of quantitative
guidelines that prescribe the minimal number of samples required to infer a
reliable GRN model. We study the temporal resolution of data vs quality of GRN
inference in order to ultimately overcome this deficit. The evolution of a
Markovian jump process model for the Ras/cAMP/PKA pathway of proteins and
metabolites in the G1 phase of the Saccharomyces cerevisiae cell cycle is
sampled at a number of different rates. For each time-series we infer a linear
regression model of the GRN using the LASSO method. The inferred network
topology is evaluated in terms of the area under the precision-recall curve
AUPR. By plotting the AUPR against the number of samples, we show that the
trade-off has a, roughly speaking, sigmoid shape. An optimal number of samples
corresponds to values on the ridge of the sigmoid
Additional file 1: of Viral and metabolic controls on high rates of microbial sulfur and carbon cycling in wetland ecosystems
Table S1. Summary of samples. Sample names in this study are corresponded to samples names in our previous 16S rRNA gene study [9]. Genomic DNA (gDNA) concentrations obtained for each sample and total DNA retrieved are presented. (XLSX 10 kb
Additional file 5: of Viral and metabolic controls on high rates of microbial sulfur and carbon cycling in wetland ecosystems
Figure S2. Redundancy analyses (RDA) of microbial and viral populations. Each gene abundance (contig RPKM value) was used as input for RDA. The genes reductive dsrA and dsrD represent candidate sulfate-reducing populations, while mcrA, candidate methanogens. Forward selection provided the variables to constrain these populations, shown in the plots and stated below each plot with their associated RDA statistics. In all plots, P7 samples are indicated by gray circles, while P8 samples by white/empty circles. (PDF 518 kb
Additional file 4: of Viral and metabolic controls on high rates of microbial sulfur and carbon cycling in wetland ecosystems
Figure S1. dsrD phylogenetic affiliation and abundance per sample. This is the expanded version of Fig. 1. The RAxML tree was constructed using 206 amino acid sequences. The gene affiliation was inferred from the best BLASTP hit. The 23 clusters in Fig. 1 are indicated here. Bolded names represent dsrD present in reconstructed genomes. The yellow, blue, and orange stars indicate dsrD in genomes represented in Fig. 2. For the heat map, dsrD-containing contig RPKM values were used as input. The statistical significance of hierarchical clustering branches is indicated by green stars (pvclust, approximately unbiased p < 0.05). (PDF 1128 kb
Additional file 3: of Viral and metabolic controls on high rates of microbial sulfur and carbon cycling in wetland ecosystems
Table S3. RPKM values for viral, reductive dsrA-, dsrD-, and mcrA-containing contigs. These tables were used as inputs for many of the analyses indicated in the Methods session. (XLSX 376 kb
Additional file 8: of Viral and metabolic controls on high rates of microbial sulfur and carbon cycling in wetland ecosystems
Table S5. Summary of microbial genomes. This table provides a summary of marker genes, completeness, contamination, and RPKM values for genomes investigated in this study. (XLSX 16 kb
Additional file 11: of Viral and metabolic controls on high rates of microbial sulfur and carbon cycling in wetland ecosystems
Table S6. Summary of viral taxonomy. Taxonomic classification and vContact-based clustering for each viral contig are provided. (XLSX 81kb
Additional file 12: of Viral and metabolic controls on high rates of microbial sulfur and carbon cycling in wetland ecosystems
Figure S6. Principal component analysis (PCA) of geochemical variables. Pore water concentrations of sulfate, sulfide, ferrous iron (Fe II), and methane were retrieved from Dalcin Martins et al. [9] and used as input values for this analysis. P7 samples are represented by black circles, while P8 samples by gray circles. (PDF 102 kb
Coral physiology and microbiome dynamics under combined warming and ocean acidification
<div><p>Rising seawater temperature and ocean acidification threaten the survival of coral reefs. The relationship between coral physiology and its microbiome may reveal why some corals are more resilient to these global change conditions. Here, we conducted the first experiment to simultaneously investigate changes in the coral microbiome and coral physiology in response to the dual stress of elevated seawater temperature and ocean acidification expected by the end of this century. Two species of corals, <i>Acropora millepora</i> containing the thermally sensitive endosymbiont C21a and <i>Turbinaria reniformis</i> containing the thermally tolerant endosymbiont <i>Symbiodinium trenchi</i>, were exposed to control (26.5°C and <i>p</i>CO<sub>2</sub> of 364 μatm) and treatment (29.0°C and <i>p</i>CO<sub>2</sub> of 750 μatm) conditions for 24 days, after which we measured the microbial community composition. These microbial findings were interpreted within the context of previously published physiological measurements from the exact same corals in this study (calcification, organic carbon flux, ratio of photosynthesis to respiration, photosystem II maximal efficiency, total lipids, soluble animal protein, soluble animal carbohydrates, soluble algal protein, soluble algal carbohydrate, biomass, endosymbiotic algal density, and chlorophyll <i>a</i>). Overall, dually stressed <i>A</i>. <i>millepora</i> had reduced microbial diversity, experienced large changes in microbial community composition, and experienced dramatic physiological declines in calcification, photosystem II maximal efficiency, and algal carbohydrates. In contrast, the dually stressed coral <i>T</i>. <i>reniformis</i> experienced a stable and more diverse microbiome community with minimal physiological decline, coupled with very high total energy reserves and particulate organic carbon release rates. Thus, the microbiome changed and microbial diversity decreased in the physiologically sensitive coral with the thermally sensitive endosymbiotic algae but not in the physiologically tolerant coral with the thermally tolerant endosymbiont. Our results confirm recent findings that temperature-stress tolerant corals have a more stable microbiome, and demonstrate for the first time that this is also the case under the dual stresses of ocean warming and acidification. We propose that coral with a stable microbiome are also more physiologically resilient and thus more likely to persist in the future, and shape the coral species diversity of future reef ecosystems.</p></div
NMDS ordination using microbial OTU community composition from all 24 coral fragments.
<p>Gray vector overlay shows the proportional influence of each physiology variable to the NMDS plot distribution. Am = <i>Acropora millepora</i> (circles), Tr = <i>Turbinaria reniformis</i> (open squares), Control (blue) = 26.5°C and <i>p</i>CO<sub>2</sub> of 364 μatm, Treatment (black) = 29.0°C and <i>p</i>CO<sub>2</sub> of 750 μatm. Calc = calcification rate during the last two weeks of the study, a_cells = endosymbiotic algal cell density, Chla = chlorophyll <i>a</i> concentration, carbs = carbohydrate concentration, lipid = total lipid concentration, protein = soluble protein concentration, POC = particulate organic carbon flux, h = animal host, a = endosymbiotic algae.</p